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大肠杆菌 2

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Detection of Escherichia coli in wastewater based on enzyme immunoassay

XI Haiyan, CAI Qiang, HE Miao, SHI Hanchang

《环境科学与工程前沿（英文）》 2007年 第1卷 第3期   页码 381-384 doi: 10.1007/s11783-007-0065-6

摘要： This research describes a fast detection method on the basis of enzyme-linked immunosorbent assay (ELISA) for in drainage of wastewater treatment plants. Optimized conditions such as the reaction format (sandwich or direct), the concentrations of diluted horse radish peroxidase (HRP)- conjugate, and anti-HPR antibody and pretreatment of were studied. Those results showed that the linear range of detection for was 10 cfu/mL– 6 × 10 cfu/mL. Compared with conventional methods, it is a convenient and sensitive detection method with low cost.

关键词： conventional     sandwich     pretreatment     enzyme-linked immunosorbent     detection    

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italic>rpoS deficiency suppresses acetate accumulation in glucose-enriched culture of Escherichiacoli under an aerobic condition

Prayoga SURYADARMA, Yoshihiro OJIMA, Yuto FUKUDA, Naohiro AKAMATSU, Masahito TAYA

《化学科学与工程前沿（英文）》 2012年 第6卷 第2期   页码 152-157 doi: 10.1007/s11705-012-1287-0

摘要： The role of on the central carbon metabolism was investigated through analyzing the deficiency of this regulon gene under aerobic and glucose-enriched culture conditions. The experimental results showed that while the wild type cells exhibited an overflow metabolism effect, the -deleting mutation alleviated this effect with the significant suppression of acetate accumulation under a high glucose condition. This gene deletion also induced the twofold upregulation of and one-tenth downregulation of , respectively. The overflow metabolism effect was confirmed to be recovered by re-introducing gene into the mutant. These results demonstrated changed the central carbon metabolism toward acetate overflow through dehydrogenation of pyruvate and reduction of TCA cycle activity.

关键词： Escherichia coli     rpoS     aerobic and glucose-enriched culture     overflow metabolism    

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Enhanced disinfection of Escherichia coli and bacteriophage

Guiying RAO, Kristen S. BRASTAD, Qianyi ZHANG, Rebecca ROBINSON, Zhen HE, Ying LI

《环境科学与工程前沿（英文）》 2016年 第10卷 第4期 doi: 10.1007/s11783-016-0854-x

摘要： A novel photocatalytic Ag-Cu-TiO nanowire membrane was fabricated. Bacteria and virus disinfection was improved by co-depositing Ag and Cu onto membrane. Synergetic photocatalytic effects and free metal ions of Ag and Cu contribute to disinfection. 7.68 log removal of and 4.02 log removal of bacteriophage MS2 were achieved. Titanium dioxide (TiO ) is a widely used photocatalyst that has been demonstrated for microorganism disinfection in drinking water. In this study, a new material with a novel structure, silver and copper loaded TiO nanowire membrane (Cu-Ag-TiO ) was prepared and evaluated for its efficiency to inactivate and bacteriophage MS2. Enhanced photo-activated bactericidal and virucidal activities were obtained by the Cu-Ag-TiO membrane than by the TiO , Ag-TiO and Cu-TiO membranes under both dark and UV light illumination. The better performance was attributed to the synergies of enhanced membrane photoactivity by loading silver and copper on the membrane and the synergistic effect between the free silver and copper ions in water. At the end of a 30 min test of dead-end filtration under 254 nm UV irradiation, the Cu-Ag-TiO membrane was able to obtain an removal of 7.68 log and bacteriophage MS2 removal of 4.02 log, which have met the US EPA standard. The free metal ions coming off the membrane have concentrations of less than 10 ppb in the water effluent, far below the US EPA maximum contaminant level for silver and copper ions in drinking water. Therefore, the photo-activated disinfection by the Cu-Ag-TiO membrane is a viable technique for meeting drinking water treatment standards of microbiological water purifiers.

关键词： Photo-activated disinfection     Titanium dioxide     Nanowire membrane     Silver     Copper    

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Characteristics of two transferable aminoglycoside resistance plasmids in

Chengjun Pu, Xiaoyan Gong, Ying Sun

《环境科学与工程前沿（英文）》 2019年 第13卷 第3期 doi: 10.1007/s11783-019-1119-2

摘要：

pRKZ3 is a non-conjugative IncQ plasmid, while pKANJ7 is a conjugative IncX plasmid.

The optimal mating time of pKANJ7 varied under different conditions.

Both of the two transferable ARPs had little impact on the growth of their hosts.

A relatively high level of fitness cost was observed for pKANJ7.

The fitness cost of ARPs depended on their hosts.

关键词： Escherichia coli     Characteristics     Aminoglycoside resistance plasmids     Transfer     Persistence    

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Analysis of antibiotic resistance of

Yangyang Yu, Xiaolin Zhu, Guanlan Wu, Chengzhi Wang, Xing Yuan

《环境科学与工程前沿（英文）》 2019年 第13卷 第3期 doi: 10.1007/s11783-019-1123-6

摘要：

The concentrations of four types of antibiotics in the Yitong River were detected.

The concentration of total coliforms in summer was higher than that in spring.

There was a seasonal difference in antibiotic resistance of E. coli.

The E. coli in the Yitong River was found to have multiple antibiotic resistance.

关键词： Yitong River     Coliform bacteria     Antibiotic resistance     Escherichia coli    

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Biomimetic Macrophage–Fe3O4@PLGA Particle-Triggered Intelligent Catalysis for Killing Multidrug-Resistant Escherichiacoli

Jieni Fu,Xiangmei Liu,Zhaoyang Li,Yufeng Zheng,Yu Zhang,Hui Jiang,Yanqin Liang,Shengli Zhu,Zhenduo Cui,Shuilin Wu,

《工程（英文）》 doi: 10.1016/j.eng.2023.05.022

摘要： Infections with multidrug-resistant (MRD) Gram-negative bacteria, such as MRD Escherichia coli (E. coli), remain a challenge due to the lack of safe antibiotics and high fatality rates under anti-infection therapies. This work presents a form of biomimetic intelligent catalysis inspired by the selective biocatalytic property of macrophages, consisting of an intelligent controlling center (a living macrophage, MΦ) and a Fenton reaction catalyst (Fe3O4@poly(lactic-co-glycolic acid) (PLGA) nanoparticles) for killing MDR E. coli without harming normal cells. The MΦ–Fe3O4@PLGA particles (i.e., the intelligent catalysis particles) exhibit selective biocatalysis activity toward MDR E. coli by producing H2O2 and lipid droplets (LDs). This process activates the lipid metabolism and glycan biosynthesis and metabolism pathways based on the result of RNA sequencing data analysis. The H2O2 further reacts with Fe3O4@PLGA to form highly toxic hydroxyl radicals (•OH), while the LDs contain antimicrobial peptides and can target MDR E. coli. The highly toxic •OH and antimicrobial peptides are shown to combat with MDR E. coli, such that the antibacterial efficiency of the MΦ–Fe3O4@PLGA particles against MDR E. coli is 99.29% ± 0.31% in vitro. More importantly, after several passages, the intelligent catalysis function of the MΦ–Fe3O4@PLGA particles is well maintained. Hence, the concept of biomimetic intelligent catalysts displays potential for treating diseases other than infections.

关键词： Multidrug-resistant Escherichia coli     Macrophage–Fe3O4@PLGA particles     Biomimetic intelligent catalysis    

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结直肠癌黏膜组织来源的大肠杆菌菌株间遗传和功能差异研究 Article

常宇骁, 李享, 丁磊, 杨超, 潘志远, 韩妮, 崔玉军, 智发朝, 杨瑞馥, 高宏,毕玉晶

《工程（英文）》 2022年 第16卷 第9期   页码 210-219 doi: 10.1016/j.eng.2021.03.028

摘要：

结直肠癌是全球第三大癌症。宏基因组学已被广泛用于基于细菌属或种水平的比较来分析肠道微生物群与结直肠癌之间的关系，为结直肠癌发展中的生态失调提供了证据。然而，这种分析并不能为我们提供菌株水平的信息，进而理解一种细菌在结直肠癌发生发展中菌株水平的作用。本文利用培养组学方法分离结直肠癌黏膜样本，并选择了158株大肠杆菌，通过系统发育分析和炎症诱导实验，揭示它们在基因组学和功能上的差异。基因组比较可以将这些菌株分为5个系统群。选择代表性菌株进行THP-1细胞（人白血病单核细胞）Transwell实验以及动物实验，结果显示不同菌株经刺激后，细胞因子水平有显著性差异。进一步的生物信息学分析揭示了不同系统群间单核苷酸多态性、基因和代谢途径的不同特征，这些结果有助于了解这些菌株之间的表型差异。细菌菌株在基因组学和功能上的差异表明，菌株水平上的功能差异可以进一步了解宿主与肠道细菌的相互作用机制。

关键词： 结直肠癌     肠道菌群     培养组学     大肠杆菌     菌株水平    

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Effect of ultraviolet irradiation and chlorination on ampicillin-resistant

Yuchen PANG,Jingjing HUANG,Jinying XI,Hongying HU,Yun ZHU

《环境科学与工程前沿（英文）》 2016年 第10卷 第3期   页码 522-530 doi: 10.1007/s11783-015-0779-9

摘要： Antibiotic resistance is a serious public health risk that may spread via potable and reclaimed water. Effective disinfection is important for inactivation of antibiotic-resistant bacteria and disruption of antibiotic resistance genes. Ampicillin is a widely prescribed antibiotic but its effectiveness is increasingly undermined by resistance. In this study, changes in ampicillin resistance for ( ) CGMCC 1.1595 were analyzed after exposure to different doses of ultraviolet (UV) or chlorine, and damage incurred by the plasmid encoding ampicillin resistance gene was assessed. We reported a greater stability in ampicillin-resistant CGMCC 1.1595 after UV irradiation or chlorination when compared with previously published data for other strains. UV irradiation and chlorination led to a shift in the mortality frequency distributions of ampicillin-resistant when subsequently exposed to ampicillin. The ampicillin hemi-inhibitory concentration (IC ) without disinfection was 3800 mg·L , and an increment was observed after UV irradiation or chlorination. The IC of ampicillin-resistant was 1.5-fold higher at a UV dose of 40 mJ·cm , and was 1.4-fold higher when exposed to 2.0 mg·L chlorine. These results indicate that UV irradiation and chlorination can potentially increase the risk of selection for strains with high ampicillin resistance. There was no evident damage to after 1–10 mg Cl ·L chlorination, while a UV dose of 80 mJ·cm yielded a damage ratio for of approximately 1.2-log. Therefore, high UV doses are required for effective disruption of antibiotic resistance genes in bacteria.

关键词： antibiotic resistance     Escherichia coli     ampicillin resistance gene     ultraviolet irradiation     chlorination    

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碳青霉烯类耐药性在人源和动物源产NDM酶大肠埃希菌间的传播 Article

沈应博, 胡付品, 王永强, 尹丹丹, 杨璐, 陈义强, 许春燕, 李基云, 蒋君瑶, 王雪杨, 付玉林, 邵东延, 刘德俊, 马藤菲, 蔡畅, 沈张奇, 王少林, 李娟, 张嵘, 柯跃斌, 吴聪明, 沈建忠, Timothy R. Walsh, 汪洋

《工程（英文）》 2022年 第15卷 第8期   页码 24-33 doi: 10.1016/j.eng.2021.07.030

摘要：

Although carbapenem use is prohibited in animals in China, carbapenem-resistant Escherichia coli (CREC), especially New Delhi metallo-β-lactamase (NDM)-producing strains, are widely prevalent in foodproducing animals. At present, the impact of livestock-associated CREC strains on human populations at the national level is unknown. Here, we conduct a retrospective cross-sectional study to investigate the prevalence of CREC from clinical settings across 22 Chinese provinces or municipalities and analyze anthropogenic factors associated with their presence. We also ascertain the blaNDM and blaKPC abundance among pig and chicken farms and present a detailed genomic framework for CREC of animal and human origin. Overall, 631/29799 (2.1%) clinical Escherichia coli (E. coli) isolates were identified as CREC. Multivariable analysis revealed that being male, an age below 1, an age between 13 and 18, provinces with greater chicken production, and provinces with higher pig production were associated with higher odds of CREC infection. In general, 73.8% (n = 45/61) of pig farms and 62.2% (n = 28/45) of chicken farms had a blaNDM abundance of 1×10－5 to 1×10－3 and 1×10－3  to 1×10－2, respectively. Among all the Chinese NDM-positive E. coli (n = 463) available at the National Center for Biotechnology Information (NCBI), the genomic analysis revealed that blaNDM-5 and IncX3 were the predominant carbapenemase gene-plasmid combination, while a highly homogeneous relationship between NDM-positive isolates from humans and animals was demonstrated at the plasmid and core genome levels. All the findings suggest frequent CREC transmission between humans and animals, indicating that further discussions on the use of antibiotics in animals and humans are needed, both in China and across the globe.

关键词： Carbapenem resistance     Escherichia coli     blaNDM-5     One Health     Antimicrobial resistance transmission    

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Deposition of copper nanoparticles on multiwalled carbon nanotubes modified with poly (acrylic acid) and their antimicrobial application in water treatment

Li SHENG,Shuhang HUANG,Minghao SUI,Lingdian ZHANG,Lei SHE,Yong CHEN

《环境科学与工程前沿（英文）》 2015年 第9卷 第4期   页码 625-633 doi: 10.1007/s11783-014-0711-8

摘要： A novel hybrid material, Cu-PAA/MWCNTs (copper nanoparticles deposited multiwalled carbon nanotubes with poly (acrylic acid) as dispersant, was prepared and expected to obtain a more effective and well-dispersed disinfection material for water treatment. X-ray energy dispersive spectroscopy (EDS), transmission electron microscopy (TEM), the X-ray fluorescence (XRF), X-ray photoelectron spectra (XPS), Fourier transform infrared spectra (FT-IR), Raman spectroscopy, and thermal gravimetric analyzer (TGA) were used to characterize the Cu-PAA/MWCNTs. ( ) was employed as the target bacteria. The cell viability determination and fluorescence imaging results demonstrated that Cu-PAA/MWCNTs possessed strong antimicrobial ability on . The deposited Cu was suggested to play an important role in the antimicrobial action of Cu-PAA/MWCNTs.

关键词： multiwalled carbon nanotubes     copper nanoparticles     antimicrobial activity     Escherichia coli (E. coil)    

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Flow cytometric assessment of the effects of chlorine, chloramine, and UV on bacteria by using nucleic acid stains and 5-cyano-2,3-ditolyltetrazolium chloride

Xuebiao Nie, Wenjun Liu, Mo Chen, Minmin Liu, Lu Ao

《环境科学与工程前沿（英文）》 2016年 第10卷 第6期 doi: 10.1007/s11783-016-0884-4

摘要： Flow cytometry based on nucleic acid stains and CTC was established and optimized. Membrane of is more resistant to chlorine/chloramine than . The metabolic activity of bacteria persisted after the cytomembranewas damaged. showed more resistance to UV irradiation than by FCM. MP-UV was a stronger inhibitor of metabolic activity than LP-UV. Flow cytometry (FCM) has been widely used in multi-parametric assessment of cells in various research fields, especially in environmental sciences. This study detected the metabolic activity of and by using an FCM method based on 5-cyano-2,3-ditolyltetrazolium chloride (CTC); the accuracy of this method was enhanced by adding SYTO 9 and 10% R2A broth. The disinfection effects of chlorine, chloramine, and UV were subsequently evaluated by FCM methods. Chlorine demonstrated stronger and faster destructive effects on cytomembrane than chloramine, and nucleic acids decomposed afterwards. The metabolic activity of the bacteria persisted after the cytomembranewas damaged as detected using CTC. Low-pressure (LP) UV or medium-pressure (MP) UV treatments exerted no significant effects on membrane permeability. The metabolic activity of the bacteria decreased with increasing UV dosage, and MP-UV was a stronger inhibitor of metabolic activity than LP-UV. Furthermore, the membrane of Gram-positive was more resistant to chlorine/chloramine than that of Gram-negative . In addition, showed higher resistance to UV irradiation than .

关键词： Flow cytometry     Escherichia coli     Staphylococcusaureus     UV     CTC     SYTO 9    

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基于同一健康策略探究老挝人、动物和环境中多黏菌素耐药性的传播特征 Article

周宇晴, Refath Farzana, Somsavanh Sihalath, Sayaphet Rattanavong, Manivanh Vongsouvath, Mayfong Mayxay, Kirsty Sands, Paul N. Newton, David A.B. Dance, Brekhna Hassan, Timothy R. Walsh

《工程（英文）》 2022年 第15卷 第8期   页码 45-56 doi: 10.1016/j.eng.2022.01.013

摘要：

This study was designed to investigate the molecular epidemiology of mobile colistin resistance (mcr) using a "One-Health" approach in Laos and to predict whether any dominant plasmid backbone and/or strain type influences the dissemination of mcr. We collected 673 samples from humans (rectal normal flora), poultry, and the environment (water, flies, birds, etc.) in Vientiane, Lao People's Democratic Republic (Laos), from May to September 2018. A total of 238 Escherichia coli (E. coli) isolated from nonduplicative samples, consisting of 98 MCR-positive E. coli (MCRPEC) ("mcr" denotes the gene encoding mobile colistin resistance, and "MCR" denotes the subsequent protein encoded by mcr) and 140 MCRnegative E. coli (MCRNEC), were characterized by phenotype and Illumina sequencing. A subset of MCRPEC was selected for MinION sequencing, conjugation assay, plasmid stability, and growth kinetics in vitro. The prevalence of MCRPEC was found to be 14.6% (98/673), with the highest prevalence in human rectal swabs (45.9% (45/98), p < 0.0001, odds ratio (OR): 0.125, 95% confidence interval (CI): 0.077–0.202). The percentages of MCRPEC from other samples were 14.3% (2/14) in dog feces, 12.0% (24/200) in flies, 11.0% (11/100) in chicken meat, 8.9% (8/90) in chicken cloacal, 8.0% (4/50) in chicken caeca, and 7.5% (4/53) in wastewater. MCRPEC was significantly more resistant to co-amoxiclav, sulfamethoxazoletrimethoprim, levofloxacin, ciprofloxacin, and gentamicin than MCRNEC (p < 0.05). Genomic analysis revealed the distribution of MCRPEC among diverse clonal types. The putative plasmid Inc types associated with mcr-1 were IncX4, IncHI2, IncP1, IncI2, and IncFIA, and those associated with mcr-3 were IncFII, IncFIA, IncFIB, IncP1, and IncR. Recovery of highly similar plasmids from both flies and other sampling sectors implied the role of flies in the dissemination of mcr-1. mcr-positive plasmids were shown to be conjugative, and a significantly high transfer rate into a hypervirulent clone ST1193 was observed. Plasmids containing mcr irrespective of Inc type were highly stable and invariably did not exert a fitness effect upon introduction into a new host. These findings signify the urgent need for a standard infection control program to radically decontaminate the source of resistance.

关键词： 多黏菌素耐药性     mcr     大肠杆菌     老挝     水平传播    

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Mobile CRISPR-Cas9 based anti-phage system in E. coli

《化学科学与工程前沿（英文）》 2022年 第16卷 第8期   页码 1281-1289 doi: 10.1007/s11705-022-2141-7

摘要： Escherichia coli is one of the most important microbial cell factories, but infection by bacteriophages in the environment may have a huge impact on its application in industrial production. Here, we developed a mobile CRISPR-Cas9 based anti-phage system for bacteriophages defense in E. coli. Two conjugative plasmids pGM1 (phosphoglucomutase 1) and pGM2 carrying one and two guide RNAs, respectively, were designed to defend against a filamentous phage. The results showed that the pGM1 and pGM2 could decrease the phage infection rate to 1.6% and 0.2% respectively in infected cells. For preventing phage infection in E. coli, the pGM2 decreased the phage infection rate to 0.1%, while pGM1 failed to block phage infection. Sequence verification revealed that point mutations in protospacer or protospacer adjacent motif sequences of the phage genome caused loss of the defense function. These results support the potential application of MCBAS in E. coli cell factories to defend against phage infections.

关键词： phage infections     anti-phage     CRISPR-Cas9     conjugative transfer     synthetic biology    

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Transport of bacterial cell (

Wei Fan, Qi Li, Mingxin Huo, Xiaoyu Wang, Shanshan Lin

《环境科学与工程前沿（英文）》 2020年 第14卷 第4期 doi: 10.1007/s11783-020-1242-0

摘要： Abstract • The recharge pond dwelling process induced changes in cell properties. • Cell properties and solution chemistry exerted confounding effect on cell transport. • E. coli cells within different recharge water displayed different spreading risks. Commonly used recharge water resources for artificial groundwater recharge (AGR) such as secondary effluent (SE), river water and rainfall, are all oligotrophic, with low ionic strengths and different cationic compositions. The dwelling process in recharge pond imposed physiologic stress on Escherichia coli (E. coli) cells, in all three types of investigated recharge water resources and the cultivation of E. coli under varying recharge water conditions, induced changes in cell properties. During adaptation to the recharge water environment, the zeta potential of cells became more negative, the hydrodynamic diameters, extracellular polymeric substances content and surface hydrophobicity decreased, while the cellular outer membrane protein profiles became more diverse. The mobility of cells altered in accordance with changes in these cell properties. The E. coli cells in rainfall recharge water displayed the highest mobility (least retention), followed by cells in river water and finally SE cells, which had the lowest mobility. Simulated column experiments and quantitative modeling confirmed that the cellular properties, driven by the physiologic state of cells in different recharge water matrices and the solution chemistry, exerted synergistic effects on cell transport behavior. The findings of this study contribute to an improved understanding of E. coli transport in actual AGR scenarios and prediction of spreading risk in different recharge water sources.

关键词： Artificial groundwater recharge     E. coli     Transport     Simulated column experiments     Modeling    

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标题 作者 时间 类型 操作

Detection of Escherichia coli in wastewater based on enzyme immunoassay

XI Haiyan, CAI Qiang, HE Miao, SHI Hanchang

期刊论文

italic>rpoS deficiency suppresses acetate accumulation in glucose-enriched culture of Escherichiacoli under an aerobic condition

Prayoga SURYADARMA, Yoshihiro OJIMA, Yuto FUKUDA, Naohiro AKAMATSU, Masahito TAYA

期刊论文

Enhanced disinfection of Escherichia coli and bacteriophage

Guiying RAO, Kristen S. BRASTAD, Qianyi ZHANG, Rebecca ROBINSON, Zhen HE, Ying LI

期刊论文

Characteristics of two transferable aminoglycoside resistance plasmids in

Chengjun Pu, Xiaoyan Gong, Ying Sun

期刊论文

Analysis of antibiotic resistance of

Yangyang Yu, Xiaolin Zhu, Guanlan Wu, Chengzhi Wang, Xing Yuan

期刊论文

Biomimetic Macrophage–Fe3O4@PLGA Particle-Triggered Intelligent Catalysis for Killing Multidrug-Resistant Escherichiacoli

Jieni Fu,Xiangmei Liu,Zhaoyang Li,Yufeng Zheng,Yu Zhang,Hui Jiang,Yanqin Liang,Shengli Zhu,Zhenduo Cui,Shuilin Wu,

期刊论文

结直肠癌黏膜组织来源的大肠杆菌菌株间遗传和功能差异研究

常宇骁, 李享, 丁磊, 杨超, 潘志远, 韩妮, 崔玉军, 智发朝, 杨瑞馥, 高宏,毕玉晶

期刊论文

Effect of ultraviolet irradiation and chlorination on ampicillin-resistant

Yuchen PANG,Jingjing HUANG,Jinying XI,Hongying HU,Yun ZHU

期刊论文

碳青霉烯类耐药性在人源和动物源产NDM酶大肠埃希菌间的传播

沈应博, 胡付品, 王永强, 尹丹丹, 杨璐, 陈义强, 许春燕, 李基云, 蒋君瑶, 王雪杨, 付玉林, 邵东延, 刘德俊, 马藤菲, 蔡畅, 沈张奇, 王少林, 李娟, 张嵘, 柯跃斌, 吴聪明, 沈建忠, Timothy R. Walsh, 汪洋

期刊论文

Deposition of copper nanoparticles on multiwalled carbon nanotubes modified with poly (acrylic acid) and their antimicrobial application in water treatment

Li SHENG,Shuhang HUANG,Minghao SUI,Lingdian ZHANG,Lei SHE,Yong CHEN

期刊论文

Flow cytometric assessment of the effects of chlorine, chloramine, and UV on bacteria by using nucleic acid stains and 5-cyano-2,3-ditolyltetrazolium chloride

Xuebiao Nie, Wenjun Liu, Mo Chen, Minmin Liu, Lu Ao

期刊论文

基于同一健康策略探究老挝人、动物和环境中多黏菌素耐药性的传播特征

周宇晴, Refath Farzana, Somsavanh Sihalath, Sayaphet Rattanavong, Manivanh Vongsouvath, Mayfong Mayxay, Kirsty Sands, Paul N. Newton, David A.B. Dance, Brekhna Hassan, Timothy R. Walsh

期刊论文

汪洋：基于“One Health”视角研究碳青霉烯耐药大肠杆菌在人和动物间的传播（2022年8月31日）

2022年10月17日

会议视频

Mobile CRISPR-Cas9 based anti-phage system in E. coli

期刊论文

Transport of bacterial cell (

Wei Fan, Qi Li, Mingxin Huo, Xiaoyu Wang, Shanshan Lin

期刊论文